Science Links Japan | Reconsideration of S-genotype Assignments, and Discovery of a New Allele Based on S-RNase PCR-RFLPs in Japanese Pear Cultivars.

Reconsideration of S-genotype Assignments, and Discovery of a New Allele Based on S-RNase PCR-RFLPs in Japanese Pear Cultivars.

Accession number;01A0355610

Title;Reconsideration of S-genotype Assignments, and Discovery of a New Allele Based on S-RNase PCR-RFLPs in Japanese Pear Cultivars.

Author; CASTILLO C (Kobe Univ., Hyogo, Jpn) TAKASAKI T (Kobe Univ., Hyogo, Jpn) SAITO T (National Inst. Fruit Tree Sci., Ibaraki, Jpn) YOSHIMURA Y (Osaka Univ., Osaka, Jpn) NORIOKA S (Osaka Univ., Osaka, Jpn) NAKANISHI T (Kobe Univ., Hyogo, Jpn)

Journal Title;Breed Sci

Journal Code:Y0311B

ISSN:1344-7610

VOL.51;NO.1;PAGE.5-11(2001)

Figure&Table&Reference;FIG.3, TBL.2, REF.21

Pub. Country;Japan

Language;English

Abstract;The S-genotype assignments in the Japanese pear cultivars, 'Akaho', 'Tanzawa', 'Kimizukawase', 'Choju', 'Ichiharawase' and 'Meigetsu' previously determined by pollination tests have raised some doubt recently. These cultivars were analyzed by S-RNase based PCR-RFLP, and their S-genotype assignments reconsidered. The assignment based on the PCR-RFLP system was in agreement with that determined previously by pollination tests in six cultivars, 'Chojuro', 'Doitsu', 'Kikusui', 'Kosui', 'Taihaku' and 'Yakumo', confirming the applicability of the system. 'Akaho', 'Tanzawa', 'Kimizukawase' and 'Choju' were analyzed and assigned as S3S5, S4S5, S1S5 and S1S5, respectively. Two of these cultivars, 'Akaho' and 'Tanzawa', were also confirmed by our pollination tests. In three cultivars, 'Ichiharawase', 'Meigetsu' and 'Heiwa'('Nijisseiki'*'Ichiharawase'), a new S-RNase fragment with a size and digestion pattern distinct from those of S1 to S7-RNases of Japanese pear was amplified. The deduced amino acid sequence in the hypervariable region of this S-RNase was quite different from those of S1 to S7-RNases. We, therefore, assigned this S-RNase as S8-RNase, and identified the S-genotypes of 'Ichiharawase', 'Meigetsu' and 'Heiwa' as S1S8, S1S8 and S4S8, respectively. (author abst.)

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