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Accession number;01A0400203
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| Title;Measurement of Luminol-Dependent Chemiluminescence on Bovine Whole Milk for Detects the Early Stage of Cows Mastitis. |
| Author;
OSANAI SATORU
(Tokken)
MATSUE TAKAHISA
(Tokken)
OTSUKA YASUYUKI
(Nippon Vet. and Anim. Sci. Univ.)
SHIGETA NORITAKA
(Nippon Vet. and Anim. Sci. Univ.)
YOSHIDA TATSUYUKI
(Nippon Vet. and Anim. Sci. Univ.)
KAWASUMI KO
(Nihon'igakugijutsusen)
YOSHIMURA ITARU
(Fujianimarufamu)
TAKAHASHI HIDEYUKI
(Minist. of Agric., For. and Fish., Natl. Inst. of Anim. Health)
SUGAWARA MORIYUKI
(Nippon Vet. and Anim. Sci. Univ.)
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Journal Title;Bulletin of Nippon Veterinary and Animal Science University
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Journal Code:S0144B
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ISSN:0373-8361
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VOL.;NO.49;PAGE.40-45(2000)
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| Figure&Table&Reference;FIG.5, REF.13 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;We investigated the short-term measurement of luminol-dependent chemiluminescence in bovine whole milk samples to detect sub-clinical mastitis. Milk samples from dairy cows were classified by somatic cell counts(SCC) in 84 quarter-milk samples into 3 groups: healthy quarter (less than 200,000 cells/ml), suspected-clnical quarter (200,000 cells/ml-500,000 cells/ml) and clinical quarter (more than 500,000 cells/ml). Phagocytic activity of polymorphonuclear leucocytes was quantitated by an opsonized-zymosan-induced luminol-enhanced chemiluminescence(CL) technique. CL was measured for 15 minutes. Quarter-milk samples with a chemiluminescence under the threshold value for the healthy quarters were found in 98.3% (56/57). Healthy quarter-milk samples, above the mean, had rising tend in SCC. This finding suggests that luminol-dependent chemiluminescence with a 15 minutes-measurement can detect a rising tend in SCC. (author abst.) |
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