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Accession number;01A0468770
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| Title;The specific regioselectivity of transfer activity of .BETA.-glucosidase from Thermus sp. and the NMR analysis of main transgalactosylation products from lactose using this enzyme. |
| Author;
AKIYAMA KUMIKO
(Showajodai Daigakuinseikatsukikokenkyuka)
TAKASE MITSUNORI
(Morinaga Milk Ind. Co., Ltd., Nutr. Sci. Lab.)
SAITO TADAO
(Tohoku Univ., Graduate School of Agricultural Sci., JPN)
OKONOGI SHIGEO
(Showajodai Daigakuinseikatsukikokenkyuka)
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Journal Title;Milk Science
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Journal Code:F0966A
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ISSN:1343-0289
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VOL.50;NO.1;PAGE.7-10(2001)
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| Figure&Table&Reference;FIG.4, REF.12 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;.BETA.-Glucosidase from an extreme thermophile, Thermus sp. Z-1, has a wide substrate specificity and a strong .BETA.-galactosidase activity. 1H-NMR analysis was performed on the structure of oligosaccharides produced in the presence of the enzyme by a transglycosilation reaction, during hydrolysis of lactose, at a high temperature. Oligosaccharides were prepared from a 0.88 M lactose solution by reacting with .BETA.-glucosidase from Thermus sp. Z-1 at the concentration of 1.4 U/ml at 70.DEG.C. for 120 min. Newly transgalactosylated oligosaccharides containing disaccharides and trisaccharides in the reaction mixture were isolated by gel filtration chromatography (Bio Gel P-2) and preparative paper chromatography (PPC), followed by an analysis with 1H-NMR. In trisaccharides, the major component was Gal.BETA.1.RAR.3Gal.BETA.1.RAR.4Glc (3'-GL) and Gal.BETA.1.RAR.6Gal.BETA.1.RAR.4Glc (6'-GL) and another oligosaccharides were also determined by 1H-NMR. These results suggested that the .BETA.-glucosidase from Thermus sp. Z-1 was characterized, preferably by the transfer of galactosyl residue at the non-reducing end by .BETA.1.RAR.3 linkage. (author abst.) |
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