Microsatellite and CAPS Markers for Ethylene-related Genes, 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Synthase and ACC Oxidase Genes, and their Variation in Melon (Cucumis melo L.).
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Accession number;01A0559235
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| Title;Microsatellite and CAPS Markers for Ethylene-related Genes, 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Synthase and ACC Oxidase Genes, and their Variation in Melon (Cucumis melo L.). |
| Author;
AKASHI Y
(Okayama Univ., Okayama, Jpn)
SHIOMI S
(Kurashiki Sakuyo Univ., Okayama, Jpn)
KUBO Y
(Okayama Univ., Okayama, Jpn)
MASUDA M
(Okayama Univ., Okayama, Jpn)
KATO K
(Okayama Univ., Okayama, Jpn)
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Journal Title;Breed Sci
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Journal Code:Y0311B
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ISSN:1344-7610
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VOL.51;NO.2;PAGE.107-112(2001)
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| Figure&Table&Reference;FIG.2, TBL.3, REF.32 |
| Pub. Country;Japan |
| Language;English |
| Abstract;To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon. (author abst.) |
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