Benzyladenine-preconditioning in Germinating Mungbean Seedlings Stimulates Axillary Buds in Cotyledonary Nodes Resulting in Multiple Shoot Regeneration.
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Accession number;01A0559239
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| Title;Benzyladenine-preconditioning in Germinating Mungbean Seedlings Stimulates Axillary Buds in Cotyledonary Nodes Resulting in Multiple Shoot Regeneration. |
| Author;
AVENIDO R A
(Nagoya Univ., Aichi, Jpn)
HATTORI K
(Nagoya Univ., Aichi, Jpn)
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Journal Title;Breed Sci
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Journal Code:Y0311B
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ISSN:1344-7610
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VOL.51;NO.2;PAGE.137-142(2001)
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| Figure&Table&Reference;FIG.1, REF.31 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Tissue culture and histological studies were done to ascertain the effects of benzyladenine (BA)-preconditioning on direct shoot regeneration from cotyledonary node explants of mungbean. The highest direct shoot regeneration (93.3 %), double-sided shoot initiation (65.0 %) and average number of shoots per explant (2.7 shoots) were obtained when Pag-asa 7 explants are excised from seedlings germinated in basal medium (BM) consisting of MS salts and B5 vitamins with 1.0 mg l-1 BA, and subsequently cultured in fresh medium of the same composition. Histological inspection of sections from germinating seeds in BM and BM+1.0 mg l-1 sampled at 1 to 4 days from germination revealed bigger and developmentally more advanced axillary shoots in BA-preconditioned seedlings, a built-in advantage which could attest to the improved regenerability in BA-treated seedling explants over the control (BM only). During shoot initiation from explants, two treatments namely doubling of the concentration BA to 2.0 mg l-1 and the addition of a non-ionic surfactant, Pluronic F-68 did not further increase the responses, but gave results comparable with those obtained using BM+1.0 mg l-1 BA alone. Conversely, a BA-thidiazuron (TDZ) combination (each at 1.0 mg l-1) when added to BM significantly reduced shoot induction to only 48.3 % possibly due to increased callusing at the nodes in 55 % of the explants. The importance and applicability of the regeneration system in tissue culture and/or genetic manipulations of mungbean and other Asiatic Vigna species is discussed. (author abst.) |
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