Science Links Japan | An Electron Microscopic Analysis of the Histopathological Similarity between a Mouse Colitis Model Induced by Yersinia enterocolitica Heat-Shock Protein 60 and Human Ulcerative Colitis.

An Electron Microscopic Analysis of the Histopathological Similarity between a Mouse Colitis Model Induced by Yersinia enterocolitica Heat-Shock Protein 60 and Human Ulcerative Colitis.

Accession number;01A0305095

Title;An Electron Microscopic Analysis of the Histopathological Similarity between a Mouse Colitis Model Induced by Yersinia enterocolitica Heat-Shock Protein 60 and Human Ulcerative Colitis.

Author; KUWAHATA NORIHIRO (Toho Univ., Omori Hosp.)

Journal Title;Journal of the Medical Society of Toho University

Journal Code:G0654A

ISSN:0040-8670

VOL.48;NO.1;PAGE.34-40(2001)

Figure&Table&Reference;FIG.9, REF.23

Pub. Country;Japan

Language;Japanese

Abstract;Recently, I developed a mouse colitis model using heat-shock protein (Hsp) 60, one of the ulcerative colitis (UC) pathogens. To determine the model's value, I conducted an ultrastructural comparison of the model with that of UC patients. I administered Hsp antigens intraperitoneally to B1OA/SgSn mice, and then observed the ultrastructure of murine intestinal tissues after various periods of administration. Colonic biopsy specimens were obtained from endoscopically-normal area of ten patients with clinically mild UC. In mice treated with Hsp antigen, I observed dilatation of intercellular spaces, pyknosis, increased cellular density, small and large vacuoles in cytoplasm, and absence of perivascular reticulum fibers in the subepithelial layer. These lesions gradually worsened with time and showed the various phases of degeneration. Apoptosis in Peyer's patches and lymphoid follicles sometimes appeared. In endoscopically-normal area of the UC patients, I observed ultrastructural findings similar to those in mice after limited Hsp antigen administration. By modifying the duration of response to Hsp antigen, this model more closely approximated UC patients at various phases. These results suggest that this improved model will be useful in examining UC treatment and prevention strategies. (author abst.)

Related Articles;