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Accession number;01A0457958
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| Title;The Effect of Immunization with Herpes Simplex Virus Glycoprotein D fused with Interluekin-2 against Murine Herpetic Keratitis. |
| Author;
INOUE TOMOYUKI
(Osakadai I Gankagakukyoshitsu)
INOUE YOSHITSUGU
(Osakadai I Gankagakukyoshitsu)
NAKAMURA TAKAO
(Osakadai I Gankagakukyoshitsu)
YOSHIDA ATSUSHI
(Osakadai I Gankagakukyoshitsu)
TANO YASUO
(Osakadai I Gankagakukyoshitsu)
SHIMOMURA YOSHIKAZU
(Kinkidai I Gankagakukyoshitsu)
FUJISAWA YUKIO
(Takeda Chem. Ind., Ltd.)
AONO AKI
(Takeda Chem. Ind., Ltd.)
HAYASHI KOZABURO
(Environ. and Health Res. Lab., City of Kobe)
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Journal Title;Journal of Japanese Ophthalmological Society
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Journal Code:Z0666A
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ISSN:0029-0203
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VOL.105;NO.4;PAGE.223-229(2001)
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| Figure&Table&Reference;FIG.5, TBL.1, REF.18 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: To evaluate the effect of vaccination with fusion protein (gD-IL-2) consisting of herpes simplex type1 (HSV-1) glycoprotein D (gD) and human interleukin-2 (IL-2), and the effect of plasmid DNA vaccine encoding gD-IL-2 against murine herpetic keratitis. Methods: Plasmid containing gD-IL-2 (pHDL-neol) was constructed, and gD-IL-2 peptide was purified. BALB/c mice were injected twice hypodermally or subconjunctivally with 1.MU.g/0.1 ml of gD-IL-2 peptide, or twice subconjunctivally with 90 .MU.g/0.05 ml of gD-IL-2 plasmid DNA. Neutralizing antibody titer and delayed-type hypersensitivity (DTH) against HSV-1 were measured. Immunized mice were challenged with CHR 3 strain of HSV-1 via the cornea. The clinical picture of epithelial and stromal keratitis was scored. Results: Stromal keratitis was inhibited in gD-IL-2 peptide-or gD-IL-2 DNA-immunized mice, but epithelial keratitis was not. It was confirmed that plasmid gD-IL-2 elicited significant serum virus neutralizing titer and DTH response. Conclusion Vaccination with gD-IL-2 was effective against herpetic keratitis. (author abst.) |
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