Role of Cyclophilin in Fruiting Body Formation of Flammulina velutipes.
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Accession number;01A0622445
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| Title;Role of Cyclophilin in Fruiting Body Formation of Flammulina velutipes. |
| Author;
ODA AKI
(Gifudai Daigakuinrengonogakukenkyuka)
NAKAI RYOSUKE
(Shinshu Univ., Fac. of Agric.)
SEN KIKUO
(Shinshu Univ., Fac. of Agric.)
KUROSAWA SHIN'ICHI
(Akita Prefectural Univ., JPN)
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Journal Title;Seibutsu Kogakkaishi
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Journal Code:G0440B
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ISSN:0919-3758
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VOL.79;NO.5;PAGE.127-132(2001)
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| Figure&Table&Reference;FIG.4, REF.13 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;To elucidate the physiological role of cyclophilin, which is expressed in large quantities in fruiting bodies of Flammulina velutipes, the effect of cyclosporin A(CsA), an inhibitor of cyclophilin, on vegetative growth and fruiting body formation was studied. CsA had little effect on hyphal growth at 25.DEG.C., but inhibited growth at 13.DEG.C. and 4.DEG.C.. Fruiting body formation, which was initiated by a temperature shift from 25.DEG.C. to 13.DEG.C. or 4.DEG.C., was also markedly delayed in the presence of CsA, indicating the stress-protein nature of cyclophilin. The results showed that cyclophilin could protect several kinds of proteins from losing their biological activity under a low temperature, by which fruit body formation was initiated. The effect of CsA on the growth of several microorganisms was investigated. They could be classified into three groups in terms of the effect of CsA: (1) Escherichia coli, whose growth was not affected at all; (2) Fusarium oxysporum, Aspergillus niger, Pleurotus ostreatus, Pleurotus cornucopiae and Pholiota nameko, whose growth was completely inhibited; and (3) F. velutipes, Schizosaccharomyces pombe and Saccharomyces cerevisiae, whose growth was inhibited to some extent. (author abst.) |
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