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Accession number;01A0695794
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| Title;Plasmid Profile and Characterization on Negative Mutants for Lactose and Citrate Metabolism Derived from Leuconostoc mesenteroides. |
| Author;
SEWAKI TOMOMITSU
(Okayama Univ., Fac. of Agric.)
TAGAWA YUJI
(Okayama Univ., Fac. of Agric.)
MIYAMOTO TAKU
(Okayama Univ., Fac. of Agric.)
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Journal Title;Milk Science
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Journal Code:F0966A
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ISSN:1343-0289
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VOL.50;NO.2;PAGE.49-54(2001)
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| Figure&Table&Reference;FIG.4, TBL.2, REF.23 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Lactose- and citrate-negative (Lac-and Cit-) mutants were isolated after the treatment of Leuconostoc mesenteroides strains 6-1-9 and OR-2 with acrldine orange and examined for their plasmid profiles and enzymatical characteristics. Lac- mutants, designated 6-1-9-1 and 6-1-9-2 were dificient a 38Mdal plasmid and they lost activities of the lactose-splitting enzyme (.BETA.-galactosidase). On the other hand, Cit- mutant (OR-2-1) missing a 15Mdal plasmid lost the citrate permease activity, although it possessed less citrase activity. (author abst.) |
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