Cytoprotective Effect of Growth Factors on Cultured Rat Hepatocytes Exposed to Cytotoxic Bile Acids: An Evaluation of Polyamine Fractions.
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Accession number;01A0695921
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| Title;Cytoprotective Effect of Growth Factors on Cultured Rat Hepatocytes Exposed to Cytotoxic Bile Acids: An Evaluation of Polyamine Fractions. |
| Author;
NAGAI H
(Toho Univ. School Of Medicine, Tokyo)
ISHII K
(Toho Univ. School Of Medicine, Tokyo)
YAMAMURO W
(Saiseikai Kanagawaken Hospital, Kanagawa)
SUMINO Y
(Toho Univ. School Of Medicine, Tokyo)
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Journal Title;Journal of the Medical Society of Toho University
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Journal Code:G0654A
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ISSN:0040-8670
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VOL.48;NO.3;PAGE.216-224(2001)
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| Figure&Table&Reference;FIG.7, REF.18 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The aim of this study was to measure polyamine (PAM) fractions to investigate the effect of hepatocyte growth factor (HGF) on cultured rat hepatocytes exposed to toxic bile acids. In one series of experiments, hepatocytes were cultured with or without chenodeoxycholate, deoxycholate or vehicle (no bile acid) in medium including either epidermal growth factor (EGF), HGF or no growth factor (control). HGF significantly suppressed the lactate dehydrogenase (LDH) leakage in media with toxic bile acids, as did EGF. HGF also significantly lowered the levels of total PAM and its constituents, putrescine (PUT) and supermidine (SPD). However, the level of supermine (SPM) increased. The effects of EGF did not significantly differ from control (no growth factor). In another series of experiments, hepatocytes were cultured with toxic bile acids or vehicle in media including varied concentrations of PUT, SPD, and SPM, and in control medium (no PAM). PUT, SPD and SPM reduced LDH leakage. Our findings suggest that HGF and EGF both have a cytoprotective effect against toxic bile acids; however, the mechanism responsible for the cytoprotective effect of HGF might differ from that of EGF. (author abst.) |
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