Vasoactive Intestinal Peptide Regulates Catecholamine Secretion in Rat PC12 Cells through the Pituitary Adenylate Cyclase Activating Polypeptide Receptor.
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Accession number;01A0901047
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| Title;Vasoactive Intestinal Peptide Regulates Catecholamine Secretion in Rat PC12 Cells through the Pituitary Adenylate Cyclase Activating Polypeptide Receptor. |
| Author;
ONOUE S
(Itoham Foods Inc., Ibaraki, Jpn)
WAKI Y
(Itoham Foods Inc., Ibaraki, Jpn)
HAMANAKA K
(Itoham Foods Inc., Ibaraki, Jpn)
YAJIMA T
(Toho Univ., Chiba, Jpn)
KASHIMOTO K
(Itoham Foods Inc., Ibaraki, Jpn)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.2;PAGE.77-82(2001)
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| Figure&Table&Reference;FIG.4, REF.23 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating polypeptide (PACAP) and glucagon are members of the same family of regulatory peptides, and stimulate catecholamine release by the cAMP-mediated signaling pathway. Most members of this peptide family modulate the expression of the tyrosine hydroxylase gene through multiple adenylate cyclase-coupled receptors. In this investigation, we examined whether these peptides exerted their effects through their specific receptors in rat pheochromocytoma cells (PC12 cells). The RT-PCR experiments clearly showed the existence of the PACAP-specific (PAC1) receptor, but amplified mRNA for either of the two VIP receptor was not detected. PACAP (6-38), a potent PAC1 receptor antagonist, at a concentration of 2*10-5 M reduced the effects of VIP (10-6 M) as well as those of PACAP (10-6 M) on catecholamine secretion from PC12 cells, but had no significant effect on the effects of glucagon (10-6 M). Therefore, we suppose that VIP acts as a neurotransmitter through a PACAP-preferring receptor in PC12 cells. (author abst.) |
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