Science Links Japan | Immunocytochemical Localization of Peptidylarginine Deiminase Type III, Trichohyalin and Deiminated Trichohyalin in Infant Rat Dorsal Skin Hair Follicle.

Immunocytochemical Localization of Peptidylarginine Deiminase Type III, Trichohyalin and Deiminated Trichohyalin in Infant Rat Dorsal Skin Hair Follicle.

Accession number;01A0901049

Title;Immunocytochemical Localization of Peptidylarginine Deiminase Type III, Trichohyalin and Deiminated Trichohyalin in Infant Rat Dorsal Skin Hair Follicle.

Author; OHSAWA T (Tokyo Metropolitan Inst. Gerontology, Tokyo, Jpn) ISHIGAMI A (Tokyo Metropolitan Inst. Gerontology, Tokyo, Jpn) AKIYAMA K (Tokyo Metropolitan Inst. Gerontology, Tokyo, Jpn) ASAGA H (Tokyo Metropolitan Inst. Gerontology, Tokyo, Jpn)

Journal Title;Biomed Res

Journal Code:Z0236B

ISSN:0388-6107

VOL.22;NO.2;PAGE.91-97(2001)

Figure&Table&Reference;FIG.3, REF.39

Pub. Country;Japan

Language;English

Abstract;Peptidylarginine deiminase type III is a posttranslational enzyme to convert arginine residues in the proteins to citrullines, and exists specifically in the hair follicle and epidermis. We studied the immunocytochemical localization of the enzyme in the infant rat dorsal skin hair follicle, then compared it with the localization of trichohyalin, a putative target protein of the enzyme, and deiminated trichohyalin, the product of the enzyme reaction. The peptidylarginine deiminase type III was found to localize in both the Huxley's and Henle's layers of the inner root sheath, in the cuticle layer at the middle bulb region up to the middle follicle region, and also in the medulla cells closed to the dermal papilla and further up the follicle in the lower follicle region. The localization of trichohyalin was similar to that of the peptidylarginine deiminase type III, except that trichohyalin was detected as granules in the medulla cells closed to the dermal papilla and further up the follicle in the lower follicle region, and not in the cuticle layer. Two-dimensional gel electrophoresis revealed that the deiminated proteins in the hair follicle detected using a monospecific antibody to the chemically modified citrulline residues were deiminated trichohyalin and its proteolytic fragments. The deiminated trichohyalin detected as deiminated proteins by this antibody was found to localize in the inner root sheath from the upper bulb region further up the follicle, and also in the medulla at the middle follicle region and further up the follicle. These findings indicated that the trichohyalin and peptidylarginine deiminase type III were coincidentally expressed, but the deimination of trichohyalin occurred after their expression during the development of the hair follicle. (author abst.)

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