Phenotype-Dependent Modulation of Elastin and Collagen Expression by Elastin Peptide Val-Pro-Gly in Chick Smooth Muscle Cells in vitro.
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Accession number;01A0626980
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| Title;Phenotype-Dependent Modulation of Elastin and Collagen Expression by Elastin Peptide Val-Pro-Gly in Chick Smooth Muscle Cells in vitro. |
| Author;
HIRANO E
(Kyusyu Inst. Technol., Fukuoka, Jpn)
TAIRA K
(Kyusyu Inst. Technol., Fukuoka, Jpn)
AKIYAMA M
(National Defense Medical Coll., Saitama, Jpn)
TANAKA N
(National Defense Medical Coll., Saitama, Jpn)
OKAMOTO K
(Kyusyu Inst. Technol., Fukuoka, Jpn)
TAJIMA S
(National Defense Medical Coll., Saitama, Jpn)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.1;PAGE.25-31(2001)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.28 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Cultured smooth muscle cells (SMCs) explanted from 20-day-old chick embryo aortas exhibited a spontaneous phenotype modulation from 'contractile' (day 1 in culture) to 'synthetic' (days 3 and 5 in culture) state on the basis of the reduced expressions of calponin and .ALPHA.-actin. The cells on day 1 (contractile state) and day 3 (synthetic state) in culture were treated with chick elastin fragment Val-Pro-Gly (VPG) and responses of cell migration and extracellular matrix (elastin and collagen) expression were compared. Maximum chemotactic activity of SMCs towards VPG was observed at the dose of 10-7 M in the synthetic phenotype but none in the contractile phenotype. By contrast, VPG increased mRNA levels of elastin and type I collagen at the dose of 10-7 M in the cells on day 1 (contractile state), but rather decreased them in the cells on day 3 (synthetic state). Biosynthesis of elastin and collagen in the cells on day 1 and day 3 essentially paralleled the change of their mRNA levels. The results indicate that the effect of elastin peptide on extracellular matrix expression and chemotactic response is dependent on the phenotypes of SMC. These findings suggest that the different responses of SMC to elastin fragments may play an important role in the fibrotic change of arteriosclerosis. (author abst.) |
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