Serine Phosphorylation of Flagellar Proteins Associated with the Motility. Activation of Hamster Spermatozoa.
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Accession number;01A0626982
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| Title;Serine Phosphorylation of Flagellar Proteins Associated with the Motility. Activation of Hamster Spermatozoa. |
| Author;
FUJINOKI M
(Univ. Tokyo, Tokyo, Jpn)
OHTAKE H
(Dokkyo Univ. School Of Medicine, Tochigi, Jpn)
OKUNO M
(Univ. Tokyo, Tokyo, Jpn)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.1;PAGE.45-58(2001)
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| Figure&Table&Reference;FIG.11, TBL.2, REF.40 |
| Pub. Country;Japan |
| Language;English |
| Abstract;It has been widely accepted that the motility of spermatozoa is regulated by phosphorylation of flagellar proteins. In order to understand the regulatory mechanisms of the motility of mammalian spermatozoa, we investigated protein phosphorylation associated with motility activation. For precise analyses, we designated four steps in motility activation. They were pre-initiation (immotile spermatozoa), initiation (calcium-independent activation), activation (calcium-dependent activation), and hyperactivation. We detected 66K, 58K, and 36K proteins as the phosphoproteins related to the motility of spermatozoa. Among them, 36K proteins were separated into two different isoelectric proteins. When the motility of the spermatozoa was changed from pre-initiation to initiation, which is independent of calcium, 66K and 58K proteins were exclusively phosphorylated. When the spermatozoa were activated from the initiation by extracellular calcium, two types of 36K proteins were phosphorylated. All these proteins were phosphorylated at the serine residues. On the basis of the present results, we propose that the motility of hamster spermatozoa may be regulated through at least two pathways of protein phosphorylation. Proteins of 66K and 58K are involved in a calcium-independent pathway to initiate the motility of spermatozoa. On the other hand, two types of 36K proteins are involved in a calcium-dependent pathway to activate the motile spermatozoa. (author abst.) |
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