Design of a molecular sensor for the second messengers.
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Accession number;01A0228989
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| Title;Design of a molecular sensor for the second messengers. |
| Author;
SUGIMOTO KENJI
(Kyoto Univ., Inst. Advanced Energy, JPN)
MAKINO KEISUKE
(Kyoto Univ., Inst. Advanced Energy, JPN)
MORII TAKASHI
(Kyoto Univ., Inst. Advanced Energy, JPN)
MORI YASUO
(National Inst. for Physiological Sciences)
IMOTO KEIJI
(National Inst. for Physiological Sciences)
INOUE TERUHIKO
(Inst. for Chem. Res., Kyoto Univ.)
SUGIURA YUKIO
(Inst. for Chem. Res., Kyoto Univ.)
OTSUKA MASAMI
(Kumamoto Univ., Fac. of Pharm. Sci.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.15th;NO.;PAGE.98-99(2000)
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| Figure&Table&Reference;FIG.1 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;We have designed a molecular sensor for Ins(1,4,5)P3 by using a PH domain derived from rat brain phospholipase C.DELTA. (PLC.DELTA.), which has been shown to bind Ins(1,4,5)P3 with high affinity and selectivity. The PH domain of PLC.DELTA. cloned from the rat brain DNA library was mutated to possess a unique cysteine at the Ins(1,4,5)P3 binding site. The purified mutant PH domains were modified with various chromophores at the cysteine residue. Isolation, modification and the results of analyzing the emission behavior and their Ins(1,4,5)P3 selectivity of the PH domain will be discussed. (author abst.) |
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