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Accession number;01A0228991
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| Title;Sensor peptides based on fluorescence resonance energy transfer. |
| Author;
YOKOYAMA KENJI
(Japan Advanced Inst. Sci. and Technol., Hokuriku)
MATSUMOTO MASAYUKI
(Japan Advanced Inst. Sci. and Technol., Hokuriku)
ISHIKAWA HIDEO
(Japan Advanced Inst. Sci. and Technol., Hokuriku)
MIYAMOTO NOBUAKI
(Japan Advanced Inst. Sci. and Technol., Hokuriku)
MORITA YASUTA
(Japan Advanced Inst. Sci. and Technol., Hokuriku)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.15th;NO.;PAGE.102-103(2000)
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| Figure&Table&Reference;FIG.2 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;We designed a sensor peptide based on FRET. A 10mer sensor peptide (GSYEADRGGC) was synthesized and modified with rhodamine B and fluorescein at the terminals. The FRET property was investigated after forming a complex with the 12mer peptides as model analyte molecules. The resulting fluorescence spectrum clearly changed by addition of the analyte peptide. The FRET from Flu to Rho was reduced by forming a complex. The FRET behavior strongly depended on sequence of the analyte peptide as well as the analyte concentration. (author abst.) |
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