Detection of Alien Genes and Analysis of their Integration Position in Transgenic Rice by Fluorescence in situ Hybridization.
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Accession number;02A0066112
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| Title;Detection of Alien Genes and Analysis of their Integration Position in Transgenic Rice by Fluorescence in situ Hybridization. |
| Author;
JIN W-W
(Wuhan Univ., Wuhan, Chn)
NING S-B
(Wuhan Univ., Wuhan, Chn)
QIN R
(Wuhan Univ., Wuhan, Chn)
HUANG S-L
(Wuhan Univ., Wuhan, Chn)
LING D-H
(South China Inst. Botany, Chinese Acad. Sci., Guangzhou, Chn)
SONG Y-C
(Wuhan Univ., Wuhan, Chn)
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Journal Title;Breed Sci
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Journal Code:Y0311B
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ISSN:1344-7610
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VOL.51;NO.4;PAGE.279-283(2001)
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| Figure&Table&Reference;FIG.1, TBL.2, REF.36 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Using fluorescence in situ hybridization (FISH), we localized transferred barnase-ps1 and pHctinG DNA sequences onto chromosomes in a group of rice transgenic plants cotransformed by microprojectile bombardment. In all of the tested rice transgenic lines, the detected cells showed 1-3 signal spots and they were located in the central and terminal regions of chromosome arms. The signals of both barnase-ps1 and pHctinG genes were mostly detected in close contact on chromosomes, which could not be resolved without using two-color in situ hybridization. In a few cells, the signals were separated from each other for barnase-ps1 and pHctinG. (author abst.) |
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