Effects of Galanin on the Proliferative Activity of Immature Rat Adrenal Gland, as Investigated by Metaphase-Arrest and PCNA-Immunostaining Techniques.
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Accession number;02A0089738
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| Title;Effects of Galanin on the Proliferative Activity of Immature Rat Adrenal Gland, as Investigated by Metaphase-Arrest and PCNA-Immunostaining Techniques. |
| Author;
ZIOLKOWSKA A
(Dep. Histology And Embryology, Poznan, Pol)
BELLONI A S
(Univ. Padua, Padua, Ita)
HOCHOL A
(Dep. Histology And Embryology, Poznan, Pol)
TREJTER M
(Dep. Histology And Embryology, Poznan, Pol)
NUSSDORFER G G
(Univ. Padua, Padua, Ita)
MALENDOWICZ L K
(Dep. Histology And Embryology, Poznan, Pol)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.3;PAGE.133-136(2001)
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| Figure&Table&Reference;FIG.2, REF.26 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The effects of galanin and its non-selective receptor antagonist (galanin-A) [D-Thr6,D-Trp8,9,15-ol]-galanin1-15 on cell proliferation in immature rat adrenal glands have been investigated by metaphase-arrest and PCNA-immunostaining techniques. Galanin-A alone or with galanin lowered PCNA index in the zona glomerulosa (ZG), without affecting it in zona fasciculata-reticularis (ZF/R) and adrenal medulla (AM). In contrast, galanin-A increased metaphase index in AM, the effect being potentiated by the simultaneous administration of galanin. Metaphase index in ZG and ZF/R was not affected. Galanin per se did not change metaphase or PCNA indexes in immature rat adrenals. The conclusion is drawn that endogenous galanin exerts a tonic control of adrenal growth in immature rat: it stimulates the entry of ZG cells in the S phase and lowers the progression of AM cells in the M phase. Our investigation also indicates that the combined use of metaphase-arrest and PCNA-immunostaining methods is essential to investigate factors modulating adrenal-cell proliferation. (author abst.) |
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