Tyrosine Phosphorylation and Dephosphorylation Associated with Motility of Hamster Spermatozoa.
|
Accession number;02A0089740
|
| Title;Tyrosine Phosphorylation and Dephosphorylation Associated with Motility of Hamster Spermatozoa. |
| Author;
FUJINOKI M
(Univ. Tokyo., Tokyo, Jpn)
OHTAKE H
(Dokkyo Univ. School Of Medicine, Tochigi, Jpn)
OKUNO M
(Univ. Tokyo., Tokyo, Jpn)
|
Journal Title;Biomed Res
|
Journal Code:Z0236B
|
ISSN:0388-6107
|
|
VOL.22;NO.3;PAGE.147-155(2001)
|
| Figure&Table&Reference;FIG.4, TBL.1, REF.33 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Although sperm motility is regulated through cAMP-dependent protein phosphorylation cascade, the mechanism remains obscure. Recently it has been suggested that cAMP-dependent tyrosine phosphorylation plays an important role in the regulatory mechanisms of sperm motility. In the present experiments, fourteen flagellar proteins that ranged from 10k-Da to 120k-Da were detected as tyrosine phosphoprotein associated with the motility of hamster spermatozoa. 100k-Da, 75k-Da, 70k-Da, 60k-Da, 45k-Da, 30k-Da and 10k-Da proteins were phosphorylated when sperm motility was activated. Among them, 30k-Da and 10k-Da proteins were phosphorylated in a cAMP-dependent manner. On the other hand, 20k-Da protein was dephosphorylated when spermatozoa were activated, but it was phosphorylated in immotile spermatozoa. When activated spermatozoa were hyperactivated, 120k-Da, 115k-Da, 80k-Da, 50k-Da 40k-Da and 16k-Da proteins were phosphorylated. Form these results, it was assumed that activation of hamster sperm motility was regulated through tyrosine phosphorylation and dephosphorylation in addition to serine phosphorylation. (author abst.) |
|
|
|
Related Articles;
|
|
