Effects of Endogenous Galanin on the Growth of Regenerating Rat Adrenal Gland, as Investigated by the Metaphase-Arrest and the PCNA-Immunostaining Techniques.
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Accession number;02A0174534
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| Title;Effects of Endogenous Galanin on the Growth of Regenerating Rat Adrenal Gland, as Investigated by the Metaphase-Arrest and the PCNA-Immunostaining Techniques. |
| Author;
ZIOLKOWSKA A
(School Of Medicine, Poznan, Pol)
TORTORELLA C
(Univ. Padua, Padua, Ita)
HOCHOL A
(School Of Medicine, Poznan, Pol)
TREJTER M
(School Of Medicine, Poznan, Pol)
NUSSDORFER G G
(Univ. Padua, Padua, Ita)
MALENDOWICZ L K
(School Of Medicine, Poznan, Pol)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.4;PAGE.211-214(2001)
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| Figure&Table&Reference;FIG.1, REF.24 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We have investigated the effects of three subcutaneous injections of 2 nmol/100 g body weight of galanin and its receptor antagonist (galanin-A) [D-Thr6,D-Trp8,9,-15-ol]-galanin1-15 on the proliferative activity of regenerating rat adrenal cortex. The metaphase-arrest and the proliferating-cell nuclear antigen (PCNA)-immunostaining techniques were used to estimate the number of M phase (metaphase index) and S phase cells (PCNA index), respectively. Galanin-A raised the metaphase index at both day 5 and day 8 of regeneration. Galanin was per se ineffective, but reversed the galanin-A effect at day 8. Neither galanin nor galanin-A changed PCNA index at day 5. Galanin evoked a moderate increase in PCNA index at day 8. Taken together, these findings indicate that endogenous galanin exerts a tonic maximal inhibitory effect on adrenal regeneration in the rat. (author abst.) |
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