Stabilization of squid myosin subfragment-1 and myofibrils by ATP-related compounds.
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Accession number;02A0246685
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| Title;Stabilization of squid myosin subfragment-1 and myofibrils by ATP-related compounds. |
| Author;
YOSHIOKA T
(Hokkaido Univ., Hokkaido, Jpn)
KONNO K
(Hokkaido Univ., Hokkaido, Jpn)
SEKI N
(Hokkaido Univ., Hokkaido, Jpn)
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Journal Title;Fish Sci
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Journal Code:L2029A
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ISSN:0919-9268
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VOL.68;NO.1;PAGE.222-226(2002)
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| Figure&Table&Reference;FIG.4, REF.15 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Adenosine triphosphatase (ATPase) inactivation of squid myosin subfragment-1 (S-1) was strongly suppressed upon addition of adenylimidophosphate (AMPPNP), an analogous compound of adenosine triphosphate (ATP); whereas suppression by adenosine diphosphate (ADP) and pyrophosphate (PPi) was not as effective. Adenylimidophosphate did not stabilize myofibrils in which myosin is stabilized by F-actin. Thus, the stabilization effect by F-actin and AMPPNP is not additive. Pyrophosphate markedly accelerated the inactivation of myofibrils through the loss of protection by F-actin. The suppressive effect of F-actin on myosin ATPase inactivation was much greater than that by AMPPNP. The stabilization effects of AMPPNP and F-actin on S-1 were almost completely lost when the medium contained approximately 1 M KCl. (author abst.) |
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