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Accession number;02A0304358
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| Title;Application of the Photosensitized Peptide Nucleic Acids for the Analysis of Geno-Typing. |
| Author;
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.16th;NO.;PAGE.4-5(2001)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Peptide Nucleic Acid(PNA) is a biopolymer in which the DNA sugar-phosphate backbone is replaced by a peptide backbone. PNAs are superior to natural nucleic acids in the points: (i) they are extremely stable to cellular nucleases and proteases and (ii) can hybridize with complementary DNA with high affinity. The substitution to photo-functionalized molecules of nucleobases have been widely examined toward the application to the biotechnology. However, the protocol for the effective preparation of PNA containing a photo-functionalized molecule at desired sites has not almost been demonstrated because photo-functionalized molecules are generally unstable under alkaline conditions. We herein report that three strategic synthetic method allowed to introduce any photo-functionalized molecules into PNA oligomers at any sites. We will also report the application of the post genomic analysis such as geno-typing, SNPs detection, and so on. (author abst.) |
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