|
Accession number;02A0304361
|
| Title;Development of cationic lipids-mediated photoresponsive transfection. |
| Author;
NAGASAKI TAKESHI
(Osaka City Univ., Grad. Sch.)
TANIGUCHI AKINOBU
(Osaka City Univ., Grad. Sch.)
WADA KATSUTOSHI
(Osaka City Univ., Grad. Sch.)
|
Journal Title;Abstracts. Symposium on Biofunctional Chemistry
|
Journal Code:L0836A
|
ISSN:
|
|
VOL.16th;NO.;PAGE.10-11(2001)
|
| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The development of safe and effective gene transfer method into mammalian cells is a fundamental goal in biotechnology. We herein synthesized various photoresponsive cationic lipid based on azobenzene in oreder to modulate DAN affinities of their cationic liposomes. Irradiation of the liposome solution of trans-KAON12 at 365nm led to a mixture containing a 43:57 (cis:trans) ratio of azobenzene isomers (cis-KAON12), while irradiation of trans-KNAN12 at 440nm led to no spectral change. Dynamic light scattering and electron microscopic experiments demonstrated that the trans-to-cis photo-isomerization in KAON12-liposome caused a membrane fusion. The gel shift assay indicates that the DNA affinity of trans-KAON12 is stronger than cis-KAON12. Transfer of the liposome/plasmid DNA (pGL3-control containing luc+) complexes into the COS-1 cells were estimated using luciferase assay. Some synthetic photoresponsive cationic liposome transfect more efficiently rather than commercially available liposome-reagents (Lipofectin). Now, work on light irradiation effect using such synthetic photoresponsive cationic liposome is in progress in our laboratory. (author abst.) |
|
|
|
Related Articles;
|
