Determination of Pheophytin a Biosynthesis during Greening of Etiolated Leaves by HPLC.
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Accession number;02A0304369
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| Title;Determination of Pheophytin a Biosynthesis during Greening of Etiolated Leaves by HPLC. |
| Author;
NAKAMURA AKIMASA
(Inst. Ind. Sci., Univ. Tokyo)
WATANABE TADASHI
(Inst. Ind. Sci., Univ. Tokyo)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.16th;NO.;PAGE.26-27(2001)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In photosynthetic light reaction of green plants, minor chlorophyll(Chl) derivatives such as pheophytin(Pheo) a, Chl a' (C132 epimer of Chl a) are indispensable for efficient photochemistry within the reaction center complexes. Biosyntheses of Chl a' and Pheo a and subsequent assembly with the reaction center proteins must be critical steps for functional assembly of PS I and PS II. For understanding the biosyntheses of Chl a' and Pheo a during assembly of PS I and PS II, accumulation of Chl a' and Pheo a during greening of etiolated leaves under illumination was determined by normal-phase HPLC, which can resolve the C173 isoprenoid isomers of Chl a, Chl a', Pheo a and protochlorophyll, possible biosynthetic intermediates of these pigments. The results showed increases of the Chl a'/Chl a and the Pheo a/Chl a ratios with prolonged illumination, indicating the biosynthesis of Chl a' and Pheo a. Distribution of the C173 isomers in Chl a' was slmilar to that of Chl a. In Pheo a, the distribution of the C173 isomers shifted toward the phytyl form by comparing with that of Chl a. This might reflect the preference of Chl aP to possible demetallation enzyme(s). (author abst.) |
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