Science Links Japan | Design and synthesis of alpha-helix bundle proteins that bind a ligand in a cavity.

Design and synthesis of alpha-helix bundle proteins that bind a ligand in a cavity.

Accession number;02A0304372

Title;Design and synthesis of alpha-helix bundle proteins that bind a ligand in a cavity.

Author; OBATAYA IKUO (Tokyo Inst. of Technology, Graduate School) UENO AKIHIKO (Tokyo Inst. of Technology, Graduate School) MIHARA HISAKAZU (Tokyo Inst. of Technology, Graduate School)

Journal Title;Abstracts. Symposium on Biofunctional Chemistry

Journal Code:L0836A

ISSN:

VOL.16th;NO.;PAGE.32-33(2001)

Figure&Table&Reference;

Pub. Country;Japan

Language;Japanese

Abstract;As a model of artificial receptor protein, de novo deslgned hetero three-.ALPHA.-helix proteins that have a cavity were constructed. Hetero trimeric helix bundles were constructed by conjugation of peptide A having two cysteines with peptide B whose side-chain of Lys is bromoacetylated. In order to detect a ligand binding, fluorescent groups were introduced in the peptide A. The ligand binding was observed by fluorescent spectrometry. (author abst.)

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