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Accession number;02A0304379
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| Title;Intracellular signals-responsive artificial gene-regulation systems. |
| Author;
KATAYAMA YOSHIKI
(Kyushu Univ., Grad. Sch.)
SAKAKIHARA SHIGEKI
(Kyushu Univ., Grad. Sch.)
SONODA TATSUHIKO
(Kyushu Univ., Grad. Sch.)
FUJII KENJI
(Kyushu Univ., Grad. Sch.)
ITO ETSUKO
(Kyushu Univ., Grad. Sch.)
MAEDA MIZUO
(Kyushu Univ., Grad. Sch.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.16th;NO.;PAGE.46-47(2001)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;DNA in eukaryotic cell takes an electrostatic interaction with the histon-complex so that the transcription is totally suppressed. When the transcription of the gene is activated, lysine residues in the histon are acetylated to weaken the electrostatic interaction. We report here a development of a novel gene regulation system that responds to protein kinase A(PKA) or caspase signals. This system was composed of a DNA and newly synthesized cationic polymers in which substrate peptides of PKA or caspase were introduced. These cationic polymers made electrostatic complexes with the DNA and inhibited the expression of the gene coded in the DNA. When activated PKA or caspase was added to the polymer-DNA complex, the DNA was released from the polymer and the gene expression was significantly restored with the phosphorylation of the polymer. This is the first example of the artificial gene regulation system that responds to intracellular signal. (author abst.) |
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