Highly Zinc-Selective Fluorescent Sensor Molecules Suitable for Biological Applications.
|
Accession number;02A0304380
|
| Title;Highly Zinc-Selective Fluorescent Sensor Molecules Suitable for Biological Applications. |
| Author;
KIKUCHI KAZUYA
(Univ. Tokyo, Graduate School, JPN)
HIRANO TOMOYA
(Univ. Tokyo, Graduate School, JPN)
MARUYAMA SATOKO
(Univ. Tokyo, Graduate School, JPN)
NAGANO TETSUO
(Univ. Tokyo, Graduate School, JPN)
|
Journal Title;Abstracts. Symposium on Biofunctional Chemistry
|
Journal Code:L0836A
|
ISSN:
|
|
VOL.16th;NO.;PAGE.48-49(2001)
|
| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Recently, the physiological significance of chelatable Zn2+ has attracted much attention. However little is known about the cellular regulation of Zn2+, so several chemical tools for measuring Zn2+ in living cells have been developed. Fluorescence probes for Zn2+ ZnAF-1 and ZnAF-2, were developed based on PET arid their experimental utility to detect Zn2+ from living cells was examined. ZnAFs were designed, utilizing the N,N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine(TPEN) moiety as the acceptor for Zn2+, linked to aminofluorescein as a fluorophore. Upon addition of Zn2+ the fluorescence intensity immediately increased. The apparent dissociation constant. Kd was in the sub nM range and the detection limit of ZnAFs is also in this range, which is sufficient for application in mammalian cells. Other cations, which exist at high concentrations under physiological conditions, did not increase the fluorescence intensity. (author abst.) |
|
|
|
Related Articles;
|
|
