Ribozyme-Mimic Using Site-Selective RNA Activation.
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Accession number;02A0304385
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| Title;Ribozyme-Mimic Using Site-Selective RNA Activation. |
| Author;
KUZUYA AKINORI
(Univ. of Tokyo, RCAST Res. Center for Adv. Sci. and Technol.)
MIZOGUCHI RYO
(Univ. of Tokyo, RCAST Res. Center for Adv. Sci. and Technol.)
KOMIYAMA MAKOTO
(Univ. of Tokyo, RCAST Res. Center for Adv. Sci. and Technol.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.16th;NO.;PAGE.58-59(2001)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The most common strategy for sequence-selective RNA hydrolysis is to tether chemical scissors to DNA oligomers which are complementary with a part of the substrate RNA. However, non-covalent systems are also important, since they are easily obtainable without complicated organic synthesis. Here we present new ribozyme-mimics which are easily prepared and can be widely used for various purposes. The present artificial enzymes are composed of (1) a DNA bearing an acridine and (2) a catalyst for RNA scission (e.g., lanthanide, Zn(II), and Mg(II) ions). When the modified DNA is bound to the substrate RNA, the phosphodiester linkage in front of the acridine is selectively activated. Thus, this linkage is preferentially hydrolyzed, even though the catalyst is not bound to any place. The scission is sufficiently prompt under physiological conditions. Compared with RNA-based ribozymes, the present ribozyme-mimics have the following advantages: (1) they are more stable against enzymatic degradation and can be made still more stable by chemical modification, (2) sterically hindered RNA, which is hardly cut by ribozymes, can be effectively hydrolyzed, and (3) various physiological functions can be easily provided by chemical modification. A number of applications either in vivo or in vitro are highly promising. (author abst.) |
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