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Accession number;02A0304386
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| Title;Recognition of DNA Sequence and Chain Length Using Reversible Association and Dispersion of DNA-linked Nanoparticles. |
| Author;
TANG Z
(Kyushu Univ., Grad. Sch.)
MORI TAKESHI
(Kyushu Univ., Grad. Sch.)
TAKARADA TOORU
(Kyushu Univ., Grad. Sch.)
MAEDA MIZUO
(Kyushu Univ., Grad. Sch.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.16th;NO.;PAGE.60-61(2001)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The amphiphilic copolymer consisting of oligodeoxyribonucleotide(ODN) as the hydrophilic part and thermo-responsive poly(N-isopropylacrylamide)(PNIPAAm) as the hydrophobic part was prepared. The copolymers formed DNA-linked colloidal nanoparticles above the phase transition temperature of PNIPAAm part. The averaged radius of the nanoparticles was estimated to be 25nm at 40.DEG.C. by dynamic light scattering measurement using cumulants method. The assembling behavior of the nanoparticles was monitored by UV measurement. The nanoparticles aggregated rapidly when the complementary DNA was added into the dispersion. In contrast, they kept dispersed in the absence of the complementary DNA and in the presence of the point-mutated DNA. These distinct phenomena may be applied for an oligonucleotides discrimination system in gene diagnosis. (author abst.) |
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