Design of a molecular sensor for the second messengers.
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Accession number;02A0304401
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| Title;Design of a molecular sensor for the second messengers. |
| Author;
SUGIMOTO KENJI
(Kyoto Univ., Inst. Advanced Energy, JPN)
MORII TAKASHI
(Kyoto Univ., Inst. Advanced Energy, JPN)
MORI YASUO
(National Inst. for Physiological Sciences)
IMOTO KEIJI
(National Inst. for Physiological Sciences)
OTSUKA MASAMI
(Kumamoto Univ., Fac. of Pharm. Sci.)
MAKINO KEISUKE
(Kyoto Univ., Inst. Advanced Energy, JPN)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.16th;NO.;PAGE.90-91(2001)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;We have designed a series of molecular sensors for Ins(1,4,5)P3 by using a PH domain derived from rat barain phospholipase C, which has been shown to bind Ins(1,4,5)P3 with high affinity and selectivity. The PH domain of PLC.DELTA. cloned from the rat brain DNA library was mutated to possess a unique cystein at the Ins(1,4,5)P3 binding site. The purified mutant PH domains were modified with various chromophores at the cystein residue. Isolation, modification and the results of analyzing the emission behavior and their Ins(1,4,5)P3 selectivity of the IP3 sensors will be discussed. (author abst.) |
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