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Accession number;02A0304405
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| Title;Colorimetric gene detection using DNA-modified nanospheres. |
| Author;
IHARA TOSHIHIRO
(Kumamoto Univ., Fac. of Eng.)
CHIKAURA YASUSHI
(Kumamoto Univ., Fac. of Eng.)
JO AKINORI
(Kumamoto Univ., Fac. of Eng.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.16th;NO.;PAGE.98-99(2001)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Complementary base pairing in nucleic acids would be useful as part of the building blocks for making structures with mesoscopic dimensions (nanoscale, typically 1 to 1,000nm), because their programmability allows us to design predetermined structures. We now present a novel method for colorimetric gene detection using the aggregation (networking) of oligonucleotide (ODN)-modified nanoparticles. ODNs were covalently immobilized onto organic nanospheres impregnated with fluorescent dyes. By adding the single-stranded DNAs that are complementary to the modified ODNs, the spheres gathered to produce aggregates by cross-linking though specific base pairing. The colors of the aggregates depending on the added DNA sequences were observed using an ordinary fluorescence microscope. Fluorescent resonanc energy transfer(FRET) between the nanoparticles also provided information about the point mutation on added DNAs. We demonstrated several benefits of these approaches for the analysis of the p53 gene. (author abst.) |
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