Ischemia-Reperfusion Liver Injury: Effect of a Nutritional Approach with K-17.22 on Hepatic Antioxidant Defense System.
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Accession number;02A0266675
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| Title;Ischemia-Reperfusion Liver Injury: Effect of a Nutritional Approach with K-17.22 on Hepatic Antioxidant Defense System. |
| Author;
MAROTTA F
(S. Giuseppe Hosp., Milano, Ita)
BERTUCCELLI J
(Sfjo & Lab., Paris, Fra)
ALBERGATI F
(Univ. Milano, Monza, Ita)
HARADA M
(Med. Corp. Harada Hospital, Tokyo, Jpn)
SAFRAN P
(Sfjo & Lab., Paris, Fra)
YANAIHARA N
(Yanaihara Inst., Shizuoka, Jpn)
IDEO G
(S. Giuseppe Hosp., Milano, Ita)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.5;PAGE.221-227(2001)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.37 |
| Pub. Country;Japan |
| Language;English |
| Abstract;In the present study we tested a natural compound, i.e. K-17.22, which is endowed by antioxidant properties and transaminases-lowering effect in HCV patients, on free radicals-related liver damage by ischemia/reperfusion injury. Wistar rats were put on a 2-week supplementation with either, A) standard diet or B) standard diet added with 30mg of K-17.22 (Yojyo-Henshiko: K-17.22, Kyotsu Inc., Tokyo, Japan). A classical ischemia/reperfusion liver model was prepared and after 60min of reperfusion, hepatic tissue blood flow was measured and the rats were sacrificed. Sham-operated rats were used as controls. A separate set of forty rats undergoing liver ischemia with or without dietary treatment were used for survival study. The following parameters were checked: liver tissue peroxides, SOD, Catalase, glutathione metabolism, hepatic tissue blood flow by H2 clearance method and radicals-trapping ability of K-17.22 by ESR. As compared to controls, after 60min of reperfusion, B group rats showed a significantly lower MDA level (P<0.05) with an overall impairment of the liver antioxidant defense system (SOD, Catalase, GSH, GSSG and GSH-Px) (P<0.001). All these parameters were less affected in the B group (P<0.05). In particular, GSH and GSH-Px reverted to normal level with a significantly lower GPT level (P<0.05). However, K-17.22 didn't show any direct free radicals-trapping ability either on superoxide nor on hydroxyl radical generation systems. Ischemia-reperfusion phenomenon brought about a nearly 40% drop of the liver blood flow in the standard diet-fed rats (P<0.001 vs. sham-operated). Pre-treatment with K-17.22 enabled a recovery of such haemodynamic parameter (P<0.05 vs. untreated group).... (author abst.) |
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