Motility-Associated and Cyclic AMP-dependent Protein Phosphorylation in the Sperm of the Chum Salmon, Oncorhynchus keta.
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Accession number;02A0266678
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| Title;Motility-Associated and Cyclic AMP-dependent Protein Phosphorylation in the Sperm of the Chum Salmon, Oncorhynchus keta. |
| Author;
ITOH A
(Univ. Tokyo, Kanagawa, Jpn)
INABA K
(Tohoku Univ., Aomori, Jpn)
FUJINOKI M
(Dokkyo Univ. School Of Medicine, Tochigi, Jpn)
MORISAWA M
(Univ. Tokyo, Kanagawa, Jpn)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.5;PAGE.241-248(2001)
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| Figure&Table&Reference;FIG.5, TBL.2, REF.30 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Cyclic AMP-dependent cell signaling pathway has been considered to play an essential role in the initiation of sperm motility in salmonid fishs. We showed here that inhibitors of cyclic AMP-dependent protein kinase blocked the motility of the sperm of chum salmon whose plasma membrane was removed with TritonX-100. Seven proteins, which were phosphorylated by cAMP concomitantly with motility initiation of the demembranated sperm, were identified. Among them two proteins were identified as 21kDa light chain of the outer arm dynein and 48kDa regulatory subunit of protein kinase-A(PKA) from their molecular masses. Five proteins had the molecular masses of 28kDa, 33kDa, 41kDa, 63kDa and 83kDa. Phosphorylation of these proteins as well as the motility of the demembranated sperm were significantly inhibited by the PKA inhibitors. When the demembranated sperm were prepared after pre-activation, all of these 7 proteins had less labeled 32P. These results suggest that cAMP-dependent phosphorylation of multiple proteins through activation of PKA is involved in the initiation of sperm motility in the salmonid fish. (author abst.) |
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