Calcium inflow of isolated single Merkel cells in response to direct mechanical stimulation.
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Accession number;02A0266680
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| Title;Calcium inflow of isolated single Merkel cells in response to direct mechanical stimulation. |
| Author;
TAZAKI M
(Tokyo Dental Coll., Chiba, Jpn)
TAZAKI Y
(Tokyo Dental Coll., Chiba, Jpn)
INOUE T
(Tokyo Dental Coll., Chiba, Jpn)
SHIMONO M
(Tokyo Dental Coll., Chiba, Jpn)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.5;PAGE.253-256(2001)
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| Figure&Table&Reference;FIG.2, REF.12 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The changes in intracellular Ca2+ concentration ([Ca2+]i) of single Merkel cells as a result of direct mechanical stimulation to the Merkel cell by mean of microfluorimetric techniques were investigated in order to verify the existence of mechanically-gated channels in this cell membrane. Touch domes of 4-8 week old golden hamsters were used and single Merkel cells were enzymatically isolated. Increases in [Ca2+]i of single Merkel cells were induced by direct mechanical stimulation in normal Krebs solution containing 2.5mM Ca2+. The increase in [Ca2+]i ranged from 25 to 156nM (n=7). This study revealed a probable existence of mechanically-gated channels on the Merkel cell membrane in the hamster cheek pouch mucosa and these findings support the hypothesis that the Merkel cell is the actual mechano-electric transducer. (author abst.) |
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