Development of enzyme immunoassay for equine chromogranin A.
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Accession number;02A0266682
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| Title;Development of enzyme immunoassay for equine chromogranin A. |
| Author;
NAGASAWA S
(Yanaihara Inst. Inc., Fujinomiya-shi, Jpn)
KANNO T
(Yanaihara Inst. Inc., Fujinomiya-shi, Jpn)
SATO F
(Japan Racing Assoc., Utsunomiya, Jpn)
JUN L
(Yanaihara Inst. Inc., Fujinomiya-shi, Jpn)
KATO I
(Yanaihara Inst. Inc., Fujinomiya-shi, Jpn)
YANAIHARA N
(Yanaihara Inst. Inc., Fujinomiya-shi, Jpn)
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Journal Title;Biomed Res
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Journal Code:Z0236B
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ISSN:0388-6107
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VOL.22;NO.5;PAGE.261-264(2001)
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| Figure&Table&Reference;FIG.2, TBL.1, REF.25 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A region-specific enzyme immunoassay(EIA) system for equine chromogranin A(CgA) was developed using synthetic equine CgA (335-365), based on the amino acid sequence reported by Sato et al. (19). For developing the assay system, we used anti-equine CgA (335-365) serum raised in a rabbit, biotinyl-glycilglycil-equine CgA (335-365) as labeled antigen, and synthetic equine CgA (335-365) as standard. Standard displacement curve of this assay system was paralleled with the dilution curves of equine plasma and saliva. The minimum detection limit of the assay system was approximately 10fmol/mL. The CgA-like immunoreactivity (IR-CgA) level of normal equine plasma and saliva were 0.38.+-.0.08pmol/mL and 0.25.+-.0.15pmol/mL, respectively. Gel filtration analysis of equine plasma and saliva on sephadex G75 column using PBS as eluent revealed the existence of major IR-CgA molecule having approximately 60kDa. These results indicate that the present EIA system may be of great use for the measurement of IR-CgA in equine plasma and saliva. (author abst.) |
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