Maintenance and Characterization of an Epstein Barr Virus-infected CD56-negative T Cell Lymphoma.
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Accession number;02A0169408
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| Title;Maintenance and Characterization of an Epstein Barr Virus-infected CD56-negative T Cell Lymphoma. |
| Author;
MAEKAWA I
(Univ. Tokyo, Tokyo)
SATOH H
(Univ. Tokyo, Tokyo)
AOKI N
(Tokyo Metropolitan Res. Lab. Public Health, Tokyo)
MORISHITA Y
(Univ. Tokyo, Tokyo)
TSUKAMOTO N
(Gunma Univ. School Of Medicine, Gunma)
KARASAWA M
(Gunma Univ. School Of Medicine, Gunma)
NONAKA Y
(Tokyo Police Hospital, Tokyo)
NOJIMA Y
(Gunma Univ. School Of Medicine, Gunma)
MORI S
(Univ. Tokyo, Tokyo)
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Journal Title;Jpn J Cancer Res
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Journal Code:F0633A
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ISSN:0910-5050
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VOL.93;NO.1;PAGE.61-69(2002)
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| Figure&Table&Reference;FIG.6, REF.37 |
| Pub. Country;Japan |
| Language;English |
| Abstract;T cell lymphoma carrying Epstein Barr virus (EBV+TL) is very rare among Western countries while it is much more common among Japanese. Here we report an EBV+TL which has been maintained for years by the use of mice with severe combined immune deficiency (SCID) mice. Lymphoma was obtained from a 55-year-old male suffering from oculomotor nerve palsy and lymphadenopathy. A small piece of biopsied tumor was transplanted into SCID mice and the lymphoma has been maintained for over 3 years with passages every 2-3 weeks. The maintained lymphoma, termed as TMS24, and the original lymphoma cells showed identical phenotype and genotype, including diffuse medium-sized cell morphology lacking granules, suppressor/cytotoxic immunophenotype and identical T cell receptor .BETA.-chain gene rearrangement mode. Further, both were shown to carry an identical EBV clone in terms of the number of terminal repeats and the latency II-type restricted gene expression profile. Cytogenetically, TMS24 retained two characteristic chromosomal translocations of t(1;18)(q32;q21) and t(6;12)(p21;q24). Since only one cell line with such characters has been reported previously, TMS24 should be useful for detailed analysis of EBV+TL. (author abst.) |
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