Overexpression of Urokinase-type Plasminogen Activator in Human Gastric Cancer Cell Line (AGS) Induces Tumorigenicity in Severe Combined Immunodeficient Mice.
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Accession number;02A0236718
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| Title;Overexpression of Urokinase-type Plasminogen Activator in Human Gastric Cancer Cell Line (AGS) Induces Tumorigenicity in Severe Combined Immunodeficient Mice. |
| Author;
CHOI Y-K
(Korea Res. Inst. Biosci. And Biotechnol., Taejon, Kor)
YOON B-I
(Virginia Commonwealth Univ., Virginia, Usa)
KOOK Y-H
(Seoul National Univ., Seoul, Kor)
WON Y-S
(Korea Res. Inst. Biosci. And Biotechnol., Taejon, Kor)
KIM J-H
(Seoul National Univ. And School Of Agricultural Biotechnol., Suwon, Kor)
LEE C-H
(Korea Res. Inst. Biosci. And Biotechnol., Taejon, Kor)
OH G-T
(Korea Res. Inst. Biosci. And Biotechnol., Taejon, Kor)
SIPLEY J
(State Univ. New York, New York, Usa)
KIM D-Y
(Seoul National Univ. And School Of Agricultural Biotechnol., Suwon, Kor)
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Journal Title;Jpn J Cancer Res
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Journal Code:F0633A
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ISSN:0910-5050
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VOL.93;NO.2;PAGE.151-156(2002)
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| Figure&Table&Reference;FIG.8, REF.26 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The significance of urokinase-type plasminogen activator (uPA) expression in gastric cancer development was tested by using a human uPA cDNA transfection approach and an in vivo severe combined immunodeficient (SCID) mouse model. The AGS gastric cancer cell line, which has urokinase-type plasminogen-activator receptor (uPAR) but lacks uPA, was transfected with a plasmid containing human uPA cDNA and injected into the backs of SCID mice. Compared with the parent AGS cells, uPA protein secretion in AGS-2-, AGS-4-, and AGS-8-transfected cells increased by 26.1-, 34.6-, and 4.8-fold, respectively (P<0.05). mRNA expression levels of uPA in the AGS-4 clone were much stronger than those in AGS-2 and AGS-8 clones. After the cancer cells (2*106) were injected s.c. into the SCID mice, a palpable mass was observed at the injection site at around 140 days post-injection, followed by accelerated growth of the xenograft up to 180 days post-injection only in the high uPA-producing clone (AGS-4). These results suggest that continuous and high production of uPA by tumor cells is one of the important factors reflecting the malignancy of gastric cancer cells. (author abst.) |
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