Antitumor Effect of an Adeno-associated Virus Vector Containing the Human Interferon-.BETA. Gene on Experimental Intracranial Human Glioma.
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Accession number;02A0236727
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| Title;Antitumor Effect of an Adeno-associated Virus Vector Containing the Human Interferon-.BETA. Gene on Experimental Intracranial Human Glioma. |
| Author;
YOSHIDA J
(Nagoya Univ. Graduate School Of Medicine, Nagoya)
MIZUNO M
(Nagoya Univ. Graduate School Of Medicine, Nagoya)
NAKAHARA N
(Nagoya Univ. Graduate School Of Medicine, Nagoya)
COLOSI P
(Avigen, Inc., Ca, Usa)
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Journal Title;Jpn J Cancer Res
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Journal Code:F0633A
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ISSN:0910-5050
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VOL.93;NO.2;PAGE.223-228(2002)
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| Figure&Table&Reference;FIG.4, TBL.4, REF.17 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We constructed an adeno-associated virus (AAV) vector containing the human interferon-.BETA. (HuIFN-.BETA.) gene (AAV-IFN-.BETA.) and investigated its antitumor effect against human glioma cells (U251-SP) inoculated into the brain of nude mice. Prior to this, we examined human glioma cells transduced with AAV-IFN-.BETA. using video-enhanced contrast differential interference contrast (VEC-DIC) microscopy. Infection of AAV-IFN-.BETA. induced apoptosis and secondary necrosis in human glioma cells. In in vivo experiments, we confirmed production of HuIFN-.BETA. and induction of heat-shock protein (HSP) in glioma cells transduced with AAV-IFN-.BETA.. Growth of the experimental gliomas was completely inhibited by six injections of AAV-IFN-.BETA., starting 7 days after transplantation of glioma cells. In addition, the survival of mice treated with AAV-IFN-.BETA. was remarkably prolonged. These results indicate that AAV-IFN-.BETA. induces apoptosis of glioma cells and has a strong antitumor effect in this experimental glioma model. (author abst.) |
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