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Accession number;02A0368373
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| Title;Roles of Endothelin-1 in Healing Process of Gastric Ulcers in Mice. |
| Author;
KIMURA ARATA
(Osakadai Daigakuin'igakukeikenkyuka Byotaijohonaikagaku)
TSUJI SHINGO
(Osakadai Daigakuin'igakukeikenkyuka Byotaijohonaikagaku)
KAWANO SUNAO
(Osakadai I Hokengakka Byotaiseitaijohogaku)
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Journal Title;Prog Med
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Journal Code:F0664B
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ISSN:0287-3648
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VOL.22;NO.3;PAGE.762-765(2002)
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| Figure&Table&Reference;FIG.3, REF.10 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Background and Aim: Endothelin-1(ET-1) is a vasoconstrictive peptide with a pivotal role in ulcer development. However, roles of ET-1 in gastric ulcer healing, is not elucidated. Using a neutralizing antibody against ET-1, effects of ET-1 in the healing process of gastric ulcers in mice were investigated. Methods: Acetic acid-induced ulcers were produced in stomachs of BALB/c mice. After the ulcer induction(day 0), "anti-ET-1 antibody" group received intraperitoneal injections of neutralizing anti-ET-1 antibody. The control group received the same dose of normal serum. The healing process of ulcers, and the localization of ET-1 and ETA/B receptors were analyzed. Myofibroblasts isolated from stomach were cultured with ET-1 and cell proliferation was monitored using a WST-1 assay. Results: Ulcer Size was larger in the "anti ET-1 antibody" group than in the control group. ET-1 expression was observed in gastric epithelial cells. ETA receptors were observed in myofibroblasts, Whereas ETB receptors were absent in these cells. ET-1 increased proliferation of myofibroblasts in an ETA-receptor dependent manner. Conclusion: ET-1 supports gastric ulcer healing through ETA receptors. (author abst.) |
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