Lack of Direct Involvement of 8-Hydroxy-2'-deoxyguanosine in Hypoxanthine-guanine Phosphoribosyltransferase Mutagenesis in V79 Cells Treated with N,N'-Bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic-diimide (NP-III) or Riboflavin.
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Accession number;02A0357260
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| Title;Lack of Direct Involvement of 8-Hydroxy-2'-deoxyguanosine in Hypoxanthine-guanine Phosphoribosyltransferase Mutagenesis in V79 Cells Treated with N,N'-Bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic-diimide (NP-III) or Riboflavin. |
| Author;
NAKAJIMA M
(Kanazawa Univ. Graduate School Of Medical Sci., Ishikawa)
TAKEUCHI T
(Kagoshima Univ. Fac. Medicine, Kagoshima)
OGINO K
(Kanazawa Univ. Graduate School Of Medical Sci., Ishikawa)
MORIMOTO K
(Osaka Univ. Graduate School Of Medicine, Osaka)
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Journal Title;Jpn J Cancer Res
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Journal Code:F0633A
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ISSN:0910-5050
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VOL.93;NO.3;PAGE.247-252(2002)
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| Figure&Table&Reference;TBL.2, REF.36 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The object of this study is to investigate the relationship between a typical product of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine (8OHdG), and mutagenesis in V79 cells through a molecular analysis of hypoxanthine-guanine phosphoribosyltransferase (hprt) gene mutants. We performed a direct sequencing analysis of the cDNA of mutants obtained after treatment with N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic-diimide (NP-III) or riboflavin, each of which induces the formation of 8OHdG in cellular DNA upon UVA irradiation. The frequency of mutation after both treatments was no more than 2 to 5 times the control value. A considerable number of the mutants could not be amplified by RT-PCR, and this was also the case for the control mutants. Among the mutants analyzed, deletions and a TA.RAR.AT transversion occurred predominantly. The reasons for the weak association of induction of 8OHdG with frequency of mutation and the possible mechanism of oxidative-stress-derived mutagenesis are discussed. (author abst.) |
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