Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicus.

Accession number;02A0401766
Title;Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicus.
Author; SUGAYA T (Tohoku Univ., Sendai, Jpn) IKEDA M (Tohoku Univ., Sendai, Jpn) MORI H (Ehime Prefectural Chuyo Fisheries Experimental Station, Ehime, Jpn) TANIGUCHI N (Tohoku Univ., Sendai, Jpn)
Journal Title;Fish Sci
Journal Code:L2029A
ISSN:0919-9268
VOL.68;NO.2;PAGE.299-305(2002)
Figure&Table&Reference;FIG.2, TBL.3, REF.25
Pub. Country;Japan
Language;English
Abstract;The allelic inheritance mode of microsatellite DNA markers was examined using seven copulated wild females and their offspring. Five microsatellite loci, CSPJ002*, CSPJ010*, CSPJ012*, CSPJ014*, and CSPJ015*, were used in the study. At almost all family/locus combinations, one sire was determined and distributions of genotypes in offspring were consistent with the Mendelian segregation ratio. Distributions of genotypes were consistent with the ratio after assuming a null allele at some loci. Consequently, the alleles of CSPJ002* and CSPJ012* were inherited following the Mendelian inheritance mode in every family; however, the null allele was expected in CSPJ010*, CSPJ014*, and CSPJ015* in some families. Thus, these loci should be used carefully in population genetic analysis, but siblings could be detected in the dendrograms based on unweighted pair-group method using arithmetic averages (UPMGA). (author abst.)
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