Pharmacological differences in exocytosis mechanisms between dopamine, serotonin and glutamate in rat prefrontal cortex.
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Accession number;02A0472867
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| Title;Pharmacological differences in exocytosis mechanisms between dopamine, serotonin and glutamate in rat prefrontal cortex. |
| Author;
OKADA MOTOHIRO
(Hirosakidai I Shinkeiseishin'igaku)
ZHU G
(Hirosakidai I Shinkeiseishin'igaku)
MURAKAMI TAKUYA
(Hirosakidai I Shinkeiseishin'igaku)
KAMATA AKIHISA
(Hirosakidai I Shinkeiseishin'igaku)
KAWATA YUKO
(Hirosakidai I Shinkeiseishin'igaku)
KANEKO SUNAO
(Hirosakidai I Shinkeiseishin'igaku)
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Journal Title;Annual Report of the Pharmacopsychiatry Research Foundation
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Journal Code:Y0939A
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ISSN:0286-7591
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VOL.;NO.34;PAGE.4-20(2002)
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| Figure&Table&Reference;FIG.12, REF.44 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In order to explore the mechanisms of exocytosis of glutamate and monoamine, this study determined the functional interactions among voltage-sensitive Ca2+ channels(VSCCs), protein kinases (PKs) and synaptic proteins(SNAREs) on releases of glutamate, dopamine and serotonin in prefrontal cortex(pFC), using in vivo microdialysis in freely moving rats. Basal monoamine release was regulated by two functional complexes, the N-type VSCC(N-VSCC)/PKC/syntaxin(major pathway) and the P-type VSCC(P-VSCC)/PKA/synaptobrevin(minor pathway). Ca2+-evoked monoamine release was regulated by N-VSCC/PKC/syntaxin but was unaffected by P-VSCC/PKA/synaptobrevin. Basal glutamate release was unaffected by both either functional complexes, and Ca2+-evoked stimulation unaffected glutamate release. Activators of PKA and PKC increased basal and produced Ca2+-evoked glutamate releases, but surprisingly these stimulatory actions were reduced by inhibitors of P-VSCC and synaptobrevin predominantly, and inhibitors of N-VSCC and syntaxin weakly. K+-evoked neurotransmitter release was regulated by P-VSCC/PKA/synaptobrevin(major pathway) and N-VSCC/PKC/syntaxin(minor pathway). These results indicate two possibilities regarding exocytosis of glutamate and monoamine in pFC, ie. the first is that the neurotransmitter release is composed of multiple formations each induced by a specific trigger. The other is that two functional complexes, N-VSCC/PKC/syntaxin and P-VSCC/PKA/synaptobrevin, regulate both the releases of glutamate and monoamine mainly, but the sensitivity of each neurotransmitter to these complexes is different. (author abst.) |
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