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Accession number;02A0472868
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| Title;Study on the involvement of binding factors with tau protein in its polymerization for the treatment of neurodegenerative dementia. |
| Author;
TANAKA TOSHIHISA
(Osakadai Daigakuin'igakukeikenkyuka Seishin'igaku)
YAMAMORI HIDENAGA
(Osakadai Daigakuin'igakukeikenkyuka Seishin'igaku)
WADA KENJI
(Tottoridai Noshinkeinaika)
TANAKA SHUJI
(Osakadai Daigakuin'igakukeikenkyuka Seishin'igaku)
SUZUKI HIDEO
(Osakataiikudaigakutandai)
KUDO TAKASHI
(Osakadai Daigakuin'igakukeikenkyuka Seishin'igaku)
KAMINO KOJIN
(Osakadai Daigakuin'igakukeikenkyuka Seishin'igaku)
OKOUCHI MASAYASU
(Osakadai Daigakuin'igakukeikenkyuka Seishin'igaku)
TAKEDA MASATOSHI
(Osakadai Daigakuin'igakukeikenkyuka Seishin'igaku)
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Journal Title;Annual Report of the Pharmacopsychiatry Research Foundation
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Journal Code:Y0939A
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ISSN:0286-7591
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VOL.;NO.34;PAGE.21-28(2002)
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| Figure&Table&Reference;FIG.5, REF.22 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Neurofibrillary tangles(NFTs) are neuropathological hallmarks of Alzheimer disease(AD) and abnormally hyperphosphorylated tau is the major protein component of NFTs. Accumulation of hyperphosphorylated tau protein is also observed in other neurodegenerative diseases including subacute sclerosing panencephaltis(SSPE), and SSPE is caused by infection with measles RNA virus. Polyinosinic-polycytidylic acid(pI-pC), synthetic double stranded RNA, has been often used to make virus-infected cell models. In this study the effects of pI-pC in cultured cells on the phosphorylation of tau protein were investigated. SY5Y human neuroblastoma cells were treated with 50ug/ml of pI-pC, and the cell lysates were analyzed by Western blot employing the PHF-1(Ser396/404), phosphorylation dependent anti-tau antibody. Increased phosphorylation of tau protein was observed in cells treated with pI-pC, and this effect was attenuated by SB203580, an inhibitor of p38 MAPK. Therefore the signal transduction pathway leading to activation of p38 MAPK might be involved in the phosphorylation of tau protein in this model. These findings suggest that the intracellular signal transduction pathway that involves p38 MAPK might contribute to neurodegeneration in tauopathy. (author abst.) |
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