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Accession number;02A0472884
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| Title;Regulation of dopamine D2 receptor-mediated endocytosis by Arc-binding protein endophilin. |
| Author;
SUGIURA HIROKO
(Tokyo Metrop. Inst. for Neurosci.)
IWATA KEN
(Gakushuindai Ri Seimeibunshikaken)
MATSUOKA MASATO
(Niigatadai Daigakuin'ishigakusogokenkyuka Shinkeiseibutsukaibougaku)
NORITA MASAO
(Niigatadai Daigakuin'ishigakusogokenkyuka Shinkeiseibutsukaibougaku)
HAGA TATSUYA
(Gakushuindai Ri Seimeibunshikaken)
YAMAUCHI TAKASHI
(Tokushimadai Yaku Seikagaku)
YAMAGATA KANATO
(Tokyo Metrop. Inst. for Neurosci.)
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Journal Title;Annual Report of the Pharmacopsychiatry Research Foundation
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Journal Code:Y0939A
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ISSN:0286-7591
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VOL.;NO.34;PAGE.125-132(2002)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.17 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;To help define the changes in gene expression that may underlie long-lasting behavioral effects by cocaine and amphetamine, we have characterized a novel immediate early gene product Arc. To identify interacting partners with Arc, we screened a rat hippocampal library by the two-hybrid method and isolated endophilin-3S(enph3S). Endophilin-3S lacks the N-terminal 69 amino acids of endophilin-3 (enph3L), implying that enph3S is a splice variant of enph3L. Endophilin-1 is involved in the endocytosis of synaptic vesicles. It has been proposed that endophilin-1 rather acts as an effector of endocytosis by converting membrane curvature with its lysophosphatidic acid acyl transferase(LPA-AT) activity. Here we analyzed the effects of endophilin-3 on dopamine receptor-mediated endocytosis. Unexpectedly, transfection of enph3L almost completely suppressed dopamine receptor(D2)-mediated endocytosis irrespective of its high LPA-AT activity. On the other hand, activation of dynamin-GTPase by enph3L was significantly lower than that by enph3S or an N-terminal deleted enph3D, which show much less inhibitory activities on endocytosis. Furthermore, overexpression of enph3L and dynamin in HEK293T cells induced the formation of large intracellular vesicles. These data strongly suggest that the regulation of dynamin GTPase activity by endophilin is more important for receptor-mediated endocytosis than its LPA-AT activity. (author abst.) |
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