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Accession number;02A0472885
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| Title;Protective Roles of Antioxidative Reagents on 6-Hydroxydopamine-Induced Apoptotic Neuronal Cell Death. |
| Author;
IYO MASAOMI
(Chibadai Daigakuin'igakukenkyuin Seishin'igaku(k2))
SHIMIZU EIJI
(Chibadai Daigakuin'igakukenkyuin Seishin'igaku(k2))
KOMATSU NAOYA
(Chibadai Daigakuin'igakukenkyuin Seishin'igaku(k2))
HASHIMOTO KENJI
(Chibadai Daigakuin'igakukenkyuin Seishin'igaku(k2))
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Journal Title;Annual Report of the Pharmacopsychiatry Research Foundation
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Journal Code:Y0939A
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ISSN:0286-7591
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VOL.;NO.34;PAGE.133-139(2002)
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| Figure&Table&Reference;FIG.4, REF.30 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The roles of glutathione on 6-hydroxydopamine(6OH DA)-induced apoptosis in human neuroblastoma SK-N-SH cells were investigated using DNA fragmentation enzyme-immunoassay. Reduced glutathione(GSH), but not oxidized glutathione(GSSG), protected 6-OH DA(25.MU.M)-induced apoptosis, in a dose dependent manner. Furthermore, GSH inhibited the DNA fragmentation induced by 6-OH DA(25.MU.M). Preincubation(18hrs) with the glutathione syntheses inhibitor, DL-buthionine-(S,R)-sulfoximine(BSO), significantly potentiated the toxic effects of 6-OH DA(12.5 or 25.MU.M). In contrast, preincubation(18hrs) with the precursors of glutathione, N-acetylcysteine(NAC) blocked, and L-(-)-cystine, significantly attenuated 6-OH DA(25.MU.M)-induced apoptosis. These results suggest that both exogenous and endogenous GSH might play an important role in the neuroprotective mechanism against dopamine cell death. (author abst.) |
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