Comprehensive database of gene expression in the animal model of acute psychotic state.
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Accession number;02A0472886
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| Title;Comprehensive database of gene expression in the animal model of acute psychotic state. |
| Author;
OUCHI YUTA
(Tohokudai Daigakuin'igakukeikenkyuka Saibouyakurigaku)
KUBOTA YASUHIKO
(Tohokudai Daigakuin'igakukeikenkyuka Seishinshinkeigaku)
KURAMASU ATSUO
(Tohokudai Daigakuin'igakukeikenkyuka Saibouyakurigaku)
ITO CHIHIRO
(Tohokudai Daigakuin'igakukeikenkyuka Seishinshinkeigaku)
WATANABE TAKEHIKO
(Tohokudai Daigakuin'igakukeikenkyuka Saibouyakurigaku)
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Journal Title;Annual Report of the Pharmacopsychiatry Research Foundation
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Journal Code:Y0939A
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ISSN:0286-7591
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VOL.;NO.34;PAGE.140-145(2002)
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| Figure&Table&Reference;TBL.1, REF.14 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In order to comprehensively grasp the changes of gene expression pattern after acute phencyclidine (PCP) administration, we analyzed a great number of mRNAs which were sampled from the cerebral cortex of saline(1ml/kg)-treated or PCP(10mg/kg)-treated rats using Serial Analysis of Gene Expression(SAGE). We made SAGE libraries which included 49,918 tags in the saline-treated group and 40,107 tags in the PCP-treated group. In the library of saline group, 18,636 unique genes were determined, and 15,996 unique genes were determined in that of the PCP-treated group. Comparison of these two libraries revealed up-regulated genes such as i) tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, .THETA. polypeptide, ii) protein kinase C, type I(.GAMMA. type), iii) rat 70kd heat-shock-like protein mRNA, and down-regulated genes such as i) rattus norvegicus (clone .GAMMA.-3) ATP synthase .GAMMA.-subunit(ATP5c) mRNA, ii) ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c(subunit 9), isoform 1, iii) superoxide dimutase 1. In both of the saline and PCP libraries, many genes which had not been characterized previously like Expressed Sequence Tag(EST) were found. These data may provide useful information to understand the molecular basis of drug-induced psychosis and schizophrenia. (author abst.) |
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