Identification of Asian Pear Varieties by SSR Analysis.

Accession number;02A0516235
Title;Identification of Asian Pear Varieties by SSR Analysis.
Author; KIMURA T (National Center For Seeds And Seedlings, Ibaraki, Jpn) SHI Y Z (National Inst. Fruit Tree Sci., Ibaraki, Jpn) SHODA M (National Inst. Fruit Tree Sci., Ibaraki, Jpn) KOTOBUKI K (National Inst. Fruit Tree Sci., Ibaraki, Jpn) MATSUTA N (National Inst. Fruit Tree Sci., Ibaraki, Jpn) HAYASHI T (National Inst. Fruit Tree Sci., Ibaraki, Jpn) BAN Y (National Center For Seeds And Seedlings, Ibaraki, Jpn) YAMAMOTO T (National Inst. Fruit Tree Sci., Ibaraki, Jpn)
Journal Title;Breed Sci
Journal Code:Y0311B
ISSN:1344-7610
VOL.52;NO.2;PAGE.115-121(2002)
Figure&Table&Reference;FIG.3, TBL.2, REF.30
Pub. Country;Japan
Language;English
Abstract;Sixty Asian pear accessions from 6 Pyrus species were genetically identified by 9 SSR markers with a total of 133 putative alleles. Among them, 58 varieties could be successfully differentiated except for 2 pairs of synonymous or clonal varieties. All the SSR markers produced 1 or 2 discrete amplified fragments for all the diploid accessions, whereas a triploid variety showed 3 fragments with some SSRs. The number of putative alleles ranged from 7 to 20, with an average value of 14.8. The observed heterozygosity and the power of discrimination were 0.63 and 0.91, respectively. A phenogram based on the SSR genotypes was obtained, showing 3 major groups corresponding to the Japanese, Chinese and European groups. The SSR markers were highly polymorphic and could be utilized as a reliable tool for cultivar identification in Asian pears. (author abst.)
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