|
Accession number;02A0827130
|
| Title;Relationship between aging and vitamin E. |
| Author;
TAKASAKI MASARU
(Tokyo Medical College)
YANAGAWA KIYOTAKA
(Tokyo Medical College)
SHINOZAKI KAZUSHI
(Tokyo Medical College)
FUJII HIROKO
(Tokyo Medical College)
SHIBUYA TAKESHI
(Tokyoidai Yakuridai)
TAKEDA HIROSHI
(Tokyoidai Yakuridai)
MATSUMIYA TERUHIKO
(Tokyoidai Yakuridai)
EGASHIRA TOORU
(Oitaidai Yakurigaku)
|
Journal Title;Japanese Journal of Geriatrics
|
Journal Code:Z0680A
|
ISSN:0300-9173
|
|
VOL.39;NO.5;PAGE.494-500(2002)
|
| Figure&Table&Reference;FIG.10, REF.12 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;We investigated the pathophysiological significance in biomembranes of the redox dynamics of Vitamin E(.ALPHA.-tocopherol) which is lipophilic radical scavenger related to aging or pathologic status such as non-insulin-dependent diabetes mellitus or primary hyperlipidemia. Vitamin E eliminates lipid peroxyl radicals by the peroxidation chain reaction of the membrane lipid, and it becomes Vitamin E radical. Furthermore, the Vitamin E radical becomes Vitamin E quinone which is an oxidic metabolite of Vitamin E. Therefore, it was needed to determine the .ALPHA.-tocopherol and .ALPHA.-tocopherolquinone simultaneously to evaluate the antioxidative status of .ALPHA.-tocopherol in biomembranes exactly. For this purpose, we developed the assay method for the simultaneous determination of the two substances using HPLC system. Then we applied this method to basic and clinical research. 1) For the simultaneous determination of .ALPHA.-tocopherol and .ALPHA.-tocopherolquinone, highly-sensitive measurement system by HPLC-multiple coulometric ECD was developed. This system is useful to estimate the redox dynamics of .ALPHA.-tocopherol in biomembranes. 2) The utilization rate of .ALPHA.-tocopherol in the erythrocyte membrane of 10- to 120-week-old rats was significantly increased, whereas .ALPHA.-tocopherol uptake in the erythrocyte membrane decreased age-dependently. Furthermore, a significant increase in lipid hydroperoxide content and a marked decrease in the fluidity of the erythrocyte membrane were seen with age. 3) There was a strongly significant positive correlation between age and the utilization rate of .ALPHA.-tocopherol in the erythrocyte membrane of healthy volunteers aged between 23 and 103. 4) The .ALPHA.-tocopherol uptake in erythrocyte membrane was significantly lower in elderly non-insulin-dependent diabetes mellitus patients(average 68.1 years old) than in healthy subjects(average 71.8 years old).... (author abst.) |
|
|
|
Related Articles;
|
|
