Science Links Japan | Improvement of Physicochemical Properties of N-4472. Part III. VC/N-4472 Complex Formation and Self-association in Aqueous Solution.

Improvement of Physicochemical Properties of N-4472. Part III. VC/N-4472 Complex Formation and Self-association in Aqueous Solution.

Accession number;03A0040955

Title;Improvement of Physicochemical Properties of N-4472. Part III. VC/N-4472 Complex Formation and Self-association in Aqueous Solution.

Author; ITOH K (Chiba Univ., Chiba, Jpn) TOZUKA Y (Chiba Univ., Chiba, Jpn) OGUCHI T (Chiba Univ., Chiba, Jpn) YAMAMOTO K (Chiba Univ., Chiba, Jpn)

Journal Title;Chem Pharm Bull

Journal Code:G0504A

ISSN:0009-2363

VOL.51;NO.1;PAGE.40-45(2003)

Figure&Table&Reference;FIG.10, TBL.2, REF.16

Pub. Country;Japan

Language;English

Abstract;To improve the aqueous solubility of a poorly water-soluble drug, N-[2-(3,5-di-tert-butyl-4-hydroxyphenethyl)-4,6-difluorophenyl]-N'-[4-(N-benzylpiperidyl)]urea (N-4472), organic acid/N-4472 evaporates were prepared by using succinic acid, L-tartaric acid, citric acid and L-ascorbic acid (VC). Among these evaporates, only the VC/N-4472 evaporate at a molar ratio of more than 2 (VC/N-4472) formed stable colloidal particles (with a mean particle size .LEQ.100 nm) in aqueous solution. In particular, the evaporate prepared at the molar ratio of 5 (VC/N-4472) formed a clear solution comprising fine particles with a narrow particle size distribution of 8-15 nm. On the basis of surface tension measurement, zeta potential determination and static light scattering measurement, it was conceivable that both N-4472 and VC contributed to the formation of a surface-active complex in aqueous solution, while the colloidal particles could be interpreted as the self-association product of these complexes. According to the findings on '1'H-NMR and attenuated total reflectance Fourier transform infrared spectra, it was postulated that upon combining both components at a molar ratio of 2 (VC/N-4472), a 1 : 1 association complex of N-4472 and VC was formed, whereas the higher order complexes were assumedly formed with use of a molar ratio of more than 2 (VC/N-4472). Furthermore, it was evidenced that all of these complexes were composed of amphiphilic structures comprising both hydrophobic N-4472 moiety and hydrophilic VC moiety, thereby properly accounting for surface-active property of these complexes. (author abst.)

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