Primary culture of neuroblastoma cells for the immune gene therapy using tumor vaccine.
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Accession number;02A0654448
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| Title;Primary culture of neuroblastoma cells for the immune gene therapy using tumor vaccine. |
| Author;
HIROSE IMIKO
(Univ. Tokyo, Inst. Medical Sci., Advanced Clinical Res. Center, JPN)
MUGISHIMA HIDEO
(Nihon Univ., Sch. of Med.)
MITSUI TETSUO
(Yamagata Univ.)
HOSOYA RYOTA
(St. Luke's Int. Hosp.)
KIKUCHI AKIRA
(Saitama Child. Medical Center, jPN)
HYAKUNA NOBUYUKI
(Univ. Ryukyus, Fac. Med., JPN)
SHITARA TOSHIJI
(Gunmakenshoniiryose Ketsuekishuyoka)
MAEDA MIHO
(Nippon Med. Sch.)
YAMASHITA NAOHIDE
(Univ. Tokyo, Inst. Medical Sci., Advanced Clinical Res. Center, JPN)
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Journal Title;Japanese Journal of Pediatric Oncology
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Journal Code:X0797A
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ISSN:0389-4525
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VOL.39;NO.1;PAGE.37-39(2002)
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| Figure&Table&Reference;FIG.1, TBL.1, REF.7 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;From 12 child patients of neuroblastoma at stage 4, the bone marrow (11 cases) and pleural effusion (1 case) were collected and monocyte fraction cells were cultured. In 6 cases, the proliferation of neuroblastoma cells was recognized after 7 to 14 days. In 4 cases, only fibroblasts remained after one month, and in the other 2 cases, the both cells did not proliferate. After chemotherapy, it was difficult to culture the cells. In identifying the neuroblastoma cells after the culture, optical microscopic observation and GD2 staining were simple and highly reliable. It was made clear that the culture of less than 4 generations of initial or recurrent neuroblastoma cells is relatively easy, and the possibility of the immune gene therapy with tumor vaccine was suggested. |
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